Journal: International Journal of Medical Sciences

Article Title: Intrahepatic Lymphangiogenesis Is Associated with Early Post-Hepatectomy Liver Regeneration, in Part via IL-6/STAT3 Signaling

doi: 10.7150/ijms.106849

Figure Lengend Snippet: Lymphangiogenesis decreased significantly on POD3 in the process of liver regeneration following 70% PHx. A: Representatives of immunofluorescence co-staining of LYVE-1(red) and PDPN (green) in the liver on Sham, POD3, and POD7 groups (n = 5 per group, 5 biological replicates from 5 individual animals; original magnification 100X and 200X, scale bar: 100μm). B: Statistical analysis of LVs numbers in immunofluorescence staining. C: Statistical analysis of LVs area in immunofluorescence staining. D: The expression level of VEGF-C in the liver tissues was detected on Sham, POD3, POD7 groups via qRT-PCR (n=5 per group, 5 biological replicates from 5 individual animals). These results were obtained from at least three independent experiments. Values are presented as mean ± SEM. ∗∗ p < 0.01, ∗ p < 0.05, ns > 0.05.

Article Snippet: A suspension of human LyECs was loaded onto a 12-well plate (1×10 5 cells/well) and VEGF-C (0, 10, 50 ng/mL, Cat# HY- P74474 , MedChemExpress) with additional different concentrations was added to medium for 24h.

Techniques: Immunofluorescence, Staining, Expressing, Quantitative RT-PCR

Journal: International Journal of Medical Sciences

Article Title: Intrahepatic Lymphangiogenesis Is Associated with Early Post-Hepatectomy Liver Regeneration, in Part via IL-6/STAT3 Signaling

doi: 10.7150/ijms.106849

Figure Lengend Snippet: Administration of AAV-VEGF-C stimulates lymphangiogenesis in the liver. A: The expression level of VEGF-C in the liver was detected via qRT-PCR on pre-operation (n=5 per group, 5 biological replicates from 5 individual animals). B: The expression of VEGF-C in the liver was detected via qRT-PCR on POD3 and POD7 groups (n=5 per group, 5 biological replicates from 5 individual animals). C: Representatives of immunofluorescence co-staining of LYVE-1(red) and PDPN(green) in the liver among POD3, POD7(n = 5 per group, 5 biological replicates from 5 individual animals; original magnification 100X, scale bar: 100μm). D: Statistical analysis of LVs numbers in immunofluorescence staining. E: Statistical analysis of LVs area in immunofluorescence staining. These results were obtained from at least three independent experiments. Values are presented as mean ± SEM. ∗∗ p < 0.01, ∗ p < 0.05, ns > 0.05.

Article Snippet: A suspension of human LyECs was loaded onto a 12-well plate (1×10 5 cells/well) and VEGF-C (0, 10, 50 ng/mL, Cat# HY- P74474 , MedChemExpress) with additional different concentrations was added to medium for 24h.

Techniques: Expressing, Quantitative RT-PCR, Immunofluorescence, Staining

Journal: International Journal of Medical Sciences

Article Title: Intrahepatic Lymphangiogenesis Is Associated with Early Post-Hepatectomy Liver Regeneration, in Part via IL-6/STAT3 Signaling

doi: 10.7150/ijms.106849

Figure Lengend Snippet: Lymphangiogenesis contributed to the promotion of liver repair. A: HE staining was performed to assess live injury and repair in mice (n = 5 per group, 5 biological replicates from 5 individual animals; original magnification 100X, scale bar: 100μm). B and C: Serum ALT level of the AAV-Vehicle and AAV-VEGF-C groups were detected (n = 5 per group, 5 biological replicates from 5 individual animals). D and E: Serum AST level of the AAV-Vehicle and AAV-VEGF-C groups were detected (n = 5 per group, 5 biological replicates from 5 individual animals). These results were obtained from at least three independent experiments. Values are presented as mean ± SEM. ∗∗ p < 0.01, ∗ p < 0.05, ns > 0.05.

Article Snippet: A suspension of human LyECs was loaded onto a 12-well plate (1×10 5 cells/well) and VEGF-C (0, 10, 50 ng/mL, Cat# HY- P74474 , MedChemExpress) with additional different concentrations was added to medium for 24h.

Techniques: Staining

Journal: International Journal of Medical Sciences

Article Title: Intrahepatic Lymphangiogenesis Is Associated with Early Post-Hepatectomy Liver Regeneration, in Part via IL-6/STAT3 Signaling

doi: 10.7150/ijms.106849

Figure Lengend Snippet: Lymphangiogenesis promoted promoted liver regeneration by activating of IL-6/STAT3 pathway. A: IL-6 mRNA expression levels in LyECs stimulated with VEGF-C in vitro . B: The expression of IL-6 in the liver was detected via qRT-PCR on POD3 (n=5 per group, 5 biological replicates from 5 individual animals). C-D: The relative protein level of pSTAT3 in the liver were detected via western blotting on POD3 (n=5 per group, 5 biological replicates from 5 individual animals). These results were obtained from at least three independent experiments. Values are presented as mean ± SEM. ∗∗ p < 0.01, ∗ p < 0.05, ns > 0.05.

Article Snippet: A suspension of human LyECs was loaded onto a 12-well plate (1×10 5 cells/well) and VEGF-C (0, 10, 50 ng/mL, Cat# HY- P74474 , MedChemExpress) with additional different concentrations was added to medium for 24h.

Techniques: Expressing, In Vitro, Quantitative RT-PCR, Western Blot